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KMID : 0371919900030010087
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Journal of Wonju College of Medicine
1990 Volume.3 No. 1 p.87 ~ p.100
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Abstract
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After 24 and 48 hours preservation under 12 mmHg perfusion pressure, the recovery rates of isolated canine hearts were determined. Preservation was performed in a refrigerator maintained at 4℃ with modified Wicomb´s perfusate bubbled with a mixture of 95% O₂ and 5% CO₂ using a modified air lift pefusion pump unit for 24 hour (Group Ⅰ) and 48 hours (Group Ⅱ).
The extent of myocardial recovery was evaluated using a modified isolated canine working heart perfusion model by measuring heart rate, systolic arterial pressure, left atrial pressure and cardiac output (aortic overflow+coronary effluent).
Biochemical and enzymatic assays from perfusate and electron microscopic changes of the myocardium were also studied.
The results were as floolws;
1. The heart recovery rates were 95.0% in group Ⅰ and group Ⅱ (p> 0.05)
2. Recovered systolic arterial pressures (mmHg) were 99.2% in group Ⅰ and 71.3% in group Ⅱ (p=0.01).
3. Cardiac output, which is the sum of aortic overflow and coronary leakage, were 108.9±12.8% in group Ⅰ and 100.9±23.2% in group Ⅱ.
4. CPK-MB (U/L) levels were increased 332% in group Ⅰ and 1492% in group Ⅱ respectively.
5. Electron mocroscopic findings in the myocardium of group Ⅰ revealed slight to moderate muscle cell mitochondrial edema, group Ⅰ was within the limit of reversible change, but group Ⅱ was revealed moderate edema and calcium deposition from these above results. It is suggested that the safe and effective time limitation for preservation of isolated canine heart is within 24 hours under Modified Wicomb´s solution pefusion method.
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